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KMID : 0361319840160010093
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Korean Journal of Medical Technologists
1984 Volume.16 No. 1 p.93 ~ p.98
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Biotypes of Yersinia enterocolitica
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çïýéÛ×/Oh, Hung Back
ÑÑÓÞãÕ/ðÆéÄúè/ï÷ðÈêª/ßïòå÷º/ò®úçâ×/ì°ñìÓ¹/Kim, Dae Sik/Jo, Yong Hyun/Jung, Jo Won/Suh, Jin Tae/Chi, Hyun Sook/Lee, Jung Dal
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Abstract
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We have isolated 5 strains of Yersinia enterocolitica with biotypes from 803 fecal specimens of the patients for detection of enteric bacteria from November, 1980 to April, 1981 at Kyung Hee Medical Center.
All the rectal swabs was inoculated in MacConkey, SS and Selenite broth and again streaking in MacConkey and S-S agar plates after 18 hours cultivation of Selenite broth. All the agar plates were put into the room temperature. After detecting the suspected colonies through first screen Urea agar, SIM and K.I.A. Media. It was given final identification of API Kit. all the strains were determined by Nilehn & Wauters method for biotypes. and The susceptibility tests were performed by National Committee for Clinical Laboratory Standard (N.C.C.L.S) regulation.
The typical biochemical properties of the organisms were urea and ornithine positive, dextrose (no gas) and xylose were fermentative, lactose was oxidative, and indol & V.P. were variable at the room temperature, motility was negative at 35¢¥C and positive at 25¢¥C.
The pinpoint of colorless colonies showed red color after 72 hours at room temperature. It was difficult at times to detect the organisms. because of the size and color change of the colonies.
As the result of susceptibility test, all the strains were highly susceptible to Gentamycin, Kanamycin, Amikacin, Chloramphenicol, Oxytetracycline but two strains (No 1 & 5) were susceptible to Ampicillin & Carbenicillin (Biotype 2). All the strains were resistant Cephalothin.
Three of 5 strains was identified as biotype 3 and the rest as biotype 2 by Nilehn & Wauters method.
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